of Cymbidium ensifolium var. misericors in vitro.
Chen Chang is Ph.D. and works as an assistant research fellow
in Department of botany of National Museum of Natural Science,
ON: How long have
you working with the research on manipulating cytokinin components to
promote flowering Cymbidium ensifolium var. misericors
in vitro, object of your lecture in Dijon?
CC: I have been working with Chinese Cymbidium
and in vitro flowering for more than ten years since I was a Ph.D
student and postdo.
ON: Could you explain a little about the process of manipulation
of those components?
CC: Not all the cytokinins examined have a positive
influence on in vitro flowering. Among the eight cytokinins tested,
only TDZ, 2iP and BA induced flowering of rhizomes.
ON: Cymbidium takes, at least five years from sowing to flower
however you said that you got mass precocious flowering induction and
development from the cultured rhizomes, so with this process, how long
the plant gets to flower?
CC: Precocious flowers could be developed in mass
from callus-derived rhizomes within 100 days after culturing on the
ON: In general, Cymbidium has long lasting flowers, is it the
same for those plants?
CC: The flowers open in vitro could be last around
ON:Are the flowers of the same size as those cultivate under the traditional
CC: Flowers in vitro have smaller size compared
with the pot plants. But in vitro flower had the normal organs such
as petals, sepals and column.
ON: This process can be use for any genus or species of orchids?
CC: My researches are focus on the Cymbidium.
The phenomenon of in vitro flowering can be found in other orchid but
the process should be different depend on the species
ON: Concerning Taiwan, how many genera and species are there? The most
part is terrestrial or epiphyte plants?
CC: About 101 genera with 322 species, 3 subspecies
and 5 varieties in Taiwan. (Su, H. J. 2000. Flora of Taiwan, second
edition, volume five). There are more terrestrial orchids in Taiwan.
ON: Thank you very much.
by Cheng Chang
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